PCDH-CMV-Hotag3-FKBP-mCherry-TRIM1 L250P

LOCUS      PCDH-CMV-Hotag3-FKBP-mCherry-TRIM1 L250P           9846 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2490..2499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2625..2948
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             2985..3689
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    3726..5933
                     /label=trim1 l250p
     CDS             4173..4175
                     /codon_start=1
                     /label=150 a-s
                     /translation="A"
     CDS             4215..4217
                     /codon_start=1
                     /label=160r-h
                     /translation="R"
     CDS             4422..4628
                     /codon_start=1
                     /label=C-C 233-301
                     /translation="NDRFEKLKQTLEMNLTNPVKRNSELENQMAKLIQICQQVEVNTAM
                     HEAKLMEECDELVEIIQQRKQMIA"
     misc_feature    4474
                     /label=L250P
     CDS             4743..4922
                     /codon_start=1
                     /label=cos 340-399
                     /translation="LKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFAL
                     DFSREKKLLEGLDYL"
     CDS             4764..4766
                     /codon_start=1
                     /label=347 R-Q
                     /translation="R"
     misc_feature    5943..6530
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6414..6425)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6603..6836
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6908..7029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      7048..7183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7221..7237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7245..7261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7269..7299)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7314..7335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7623..8211)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8382..9242)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9243..9347)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9821..9837
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggg cgagatcgcc aagagcctga
     2521 aggagatcgc caaatccctg aaggagattg cctggtccct gaaggaaatc gccaagtccc
     2581 tgaagggcgg cggctccgga ggctctggag gatctggcgg cagcatggga gtgcaggtgg
     2641 aaaccatctc cccaggagac gggcgcacct tccccaagcg cggccagacc tgcgtggtgc
     2701 actacaccgg gatgcttgaa gatggaaaga aatttgattc ctcccgggac agaaacaagc
     2761 cctttaagtt tatgctaggc aagcaggagg tgatccgagg ctgggaagaa ggggttgccc
     2821 agatgagtgt gggtcagaga gccaaactga ctatatctcc agattatgcc tatggtgcca
     2881 ctgggcaccc aggcatcatc ccaccacatg ccactctcgt cttcgatgtg gagcttctaa
     2941 aactggaagg cggaagtgga ggatctggag gttcaggtgg ttctgtgagc aagggcgagg
     3001 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     3061 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     3121 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     3181 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     3241 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     3301 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     3361 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     3421 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     3481 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     3541 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     3601 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     3661 ccaccggcgg catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcgg
     3721 aattcatggg tgaaagccca gcctccgtgg ttcttaatgc ctcaggagga ctattttcac
     3781 taaagatgga aacactggag tctgaattga cctgtccaat ctgcctagag ttgtttgaag
     3841 acccccttct gctcccttgt gctcacagcc tctgcttcag ctgtgcccat cgcattttgg
     3901 tatcaagctg cagctctggt gaatccattg aacccattac tgctttccag tgtcctacct
     3961 gcaggtatgt tatctcgctg aaccaccggg gcctggatgg cctcaagagg aatgtgactc
     4021 tgcagaacat tattgatcgc ttccagaagg cttcagtcag tgggcccaat tcccctagtg
     4081 agagccgccg ggaaaggact tacaggccca ccactgccat gtctagcgag cgaattgctt
     4141 gccaattctg tgagcaggac ccgccaaggg atgcagtaaa aacatgcatc acctgtgagg
     4201 tctcctactg tgaccgttgc ctgcgggcca cgcaccccaa caagaaacct ttcaccagcc
     4261 accgcctggt ggaaccagtg ccagacacac atcttcgagg gatcacctgc ctggaccatg
     4321 agaatgagaa agtgaacatg tactgtgtat ctgatgacca attgatctgt gccttatgca
     4381 aactggtggg tcgtcaccga gaccatcagg tcgcatccct gaatgatcga tttgagaaac
     4441 tcaagcaaac tctggagatg aacctcacca acccggttaa gcgcaacagc gaactagaaa
     4501 atcaaatggc caaactaata cagatctgcc agcaggttga ggtgaatact gctatgcatg
     4561 aggcaaaact tatggaagaa tgtgacgagt tggtagagat catccagcag aggaagcaaa
     4621 tgatcgctgt caaaatcaaa gagacaaagg ttatgaaact gagaaagttg gcacagcagg
     4681 ttgctaattg ccgccagtgt cttgaacggt caacagtcct catcaaccaa gctgagcata
     4741 tcctgaaaga aaatgaccag gcacggtttc tacagtctgc aaaaaatatt gctgagaggg
     4801 tcgctatggc aactgcatct tctcaagttc tgattccaga catcaatttt aatgatgcct
     4861 ttgaaaactt tgctttagat ttttccagag aaaagaaact gctagagggg ttagattatt
     4921 taacagcccc aaacccacca tctatccgag aagaactctg tactgcctcc catgacacca
     4981 ttacagtcca ctggatctcg gatgatgagt tcagcatcag ctcctatgag cttcagtaca
     5041 ccatattcac tggccaggct aacttcatca gtaagtcatg gtgtagttgg ggcctgtggc
     5101 cagagataag gaaatgtaag gaagcagtaa gctgctcaag attggccggg gcgccacgag
     5161 gcctgtataa ttcagtagac agctggatga ttgttcccaa cattaaacag aaccattaca
     5221 cagtgcatgg actccagagc gggactcgct acatcttcat cgttaaagcc ataaaccaag
     5281 ccggcagccg gaacagtgaa cctacccgac taaaaacaaa cagccaaccc tttaaattgg
     5341 atcccaaaat gactcacaag aagttgaaga tctccaatga tggattgcag atggagaagg
     5401 atgaaagctc tctaaagaag agccacaccc cagagaggtt tagtggcaca gggtgctatg
     5461 gggcagcagg aaatatattc attgacagtg gctgccacta ttgggaggtg gtcatgggtt
     5521 cctcaacatg gtatgcaatt ggcattgcct acaaatcagc tccaaagaat gaatggattg
     5581 gcaagaatgc ctcctcatgg gtcttctctc gctgcaatag taacttcgtg gtgagacaca
     5641 acaacaagga aatgctggtg gatgtgcccc cacacctgaa gcgtctgggt gtcctcctgg
     5701 attatgacaa caatatgctg tctttctatg acccagctaa ctctctccat cttcatactt
     5761 ttgatgtgac cttcattctt ccagtttgtc caacatttac aatctggaac aaatccctaa
     5821 tgatcctgtc tggcttgcct gccccagatt ttattgatta ccctgagcgg caggaatgca
     5881 actgcaggcc tcaagaatcc ccttatgttt ctgggatgaa aacctgtcat taatagggat
     5941 ccaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6001 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6061 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6121 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6181 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6241 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6301 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6361 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6421 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6481 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc cggtaccttt
     6541 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6601 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     6661 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6721 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6781 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6841 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6901 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6961 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7021 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     7081 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     7141 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     7201 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     7261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     7321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7801 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     8041 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     8101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     8161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     9001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     9061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     9121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9361 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     9421 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     9481 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     9541 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     9601 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     9661 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     9721 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     9781 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     9841 aagctg
//